v08b1 229e full length spike protein sino biological  (Sino Biological)

Journal: Cell Reports Medicine

Article Title: Systemic and mucosal immune signatures of protection against SARS-CoV-2 transmission in humans

doi: 10.1016/j.xcrm.2025.102505

Figure Lengend Snippet: Longitudinal antibody response to SARS-CoV-2, HCoV-OC43, and HCoV-229E proteins in the systemic compartment Serum from participants was analyzed at baseline and at days 3, 7, and 28 post-enrollment. (A) IgG, IgM, and IgA response for SARS-CoV-2 full-length protein and S1, S2, and RBD domains according to groups (index, positive cases, negative cases). Dots represent the GMT, and bars indicate 95% CI. (B) IgG, IgM, and IgA response for HCoV-OC43 full-length protein and S1 and S2 domains according to groups (index, positive cases, negative cases). Dots represent the GMT, and bars indicate 95% CI. (C) IgG, IgM, and IgA response for HCoV-229E full-length protein according to groups (index, positive cases, negative cases). Dots represent the GMT, and bars indicate 95% CI. ∗ p ≤ 0.05. Data on antibody were compared by multiple comparison analysis (Kruskal-Wallis test); if multiple comparison was statistically significant (∗ p ≤ 0.05), a pairwise comparison was performed and the Bonferroni correction for multiple tests was applied. Data on two-by-two comparison are shown in .

Article Snippet: 229e full-length spike protein , Sino Biological , Cat#40605-V08B.

Techniques: Comparison

Journal: Cell Reports Medicine

Article Title: Systemic and mucosal immune signatures of protection against SARS-CoV-2 transmission in humans

doi: 10.1016/j.xcrm.2025.102505

Figure Lengend Snippet: Binomial logistic regression of preexisting SARS-CoV-2, HCoV-OC43, and HCoV-229E antibody levels and the probability of infection upon SARS-CoV-2 exposure in the CIDS cohort (A) Violin plot of IgG, IgM, and IgA baseline antibody titers (log10 AUC) for SARS-CoV-2-S, HCoV-OC43-S, and HCoV-229e-S full-length proteins in index, positive cases, and negative cases groups. Median (continuous black line), and IQR (Q1–Q3) values (discontinued lines) are represented. Pairwise comparisons adjusted by the Bonferroni correction for multiple test has been performed, and significance of ∗ p < 0.05 is shown. (B–D) Scatterplot diagrams showing the inverse relationship between the predicted probability of infection (PP infection, %) and immunoglobulin levels for the indicated SARS-CoV-2 antigens: S (full-length), S1, RBD, and S2; (C) HCoV-OC43: S (full-length), S1, and S2; and (D) HCoV-22E S (full-length). Logistic regression analysis has been performed for each variable individually, and significance of ∗ p < 0.05 is shown.

Article Snippet: 229e full-length spike protein , Sino Biological , Cat#40605-V08B.

Techniques: Infection

Journal: Cell Reports Medicine

Article Title: Systemic and mucosal immune signatures of protection against SARS-CoV-2 transmission in humans

doi: 10.1016/j.xcrm.2025.102505

Figure Lengend Snippet: Multi-assay modeling of mucosal signatures of protection against SARS-CoV-2 infection Nasopharyngeal swab samples from participants were analyzed at baseline and at days 3, 7, and 28 post-enrollment. (A–H) IgG, IgM, IgA, and secretory IgA (sIgA) response for (A) SARS-CoV-2 S and (B) HCoV-OC43 S proteins according to groups (index, positive cases, and negative cases) on the upper respiratory tract. Dots represent the GMT, and bars indicate 95% CI. Fold change antibody titers against (C) SARS-CoV-2 and (D) HCoV-OC43 S protein represented as boxplot. Box indicates IQR (Q1–Q3), with horizontal line at median and whiskers representing minimum and maximum. Binomial logistic regression of pre-existing (E) SARS-CoV-2 and (F) HCoV-OC43 antibody levels and the probability of SARS-CoV-2 infection. Scatterplot diagrams show the inverse relationship between the PP infection % and anti-SARS-CoV-2 S IgG, IgM, IgA, and sIgA mucosal levels. Multivariate logistic regression model analyzing the potential protection of (G) anti-SARS-CoV-2 S and (H) anti-HCoV-OC43 S IgG, IgM, IgA, and sIgA mucosal antibody levels at enrollment. (I) Multivariate logistic regression model analyzing the potential protection of mucosal anti-S IgG SARS-CoV-2 and anti-S IgA HCoV-OC43 antibody levels at enrollment from SARS-CoV-2 infection. (J) Mixed multivariate logistic regression analysis of the potential protection of systemic (SARS-CoV-2 S1 IgG and HCoV-OC43 S2 IgM) and mucosal (SARS-CoV-2 S IgG and HCoV-OC43 S IgA) antibody levels at enrollment for SARS-CoV-2 infection. Data on antibody were compared by multiple comparison analysis (Kruskal-Wallis test); if multiple comparison was statistically significant (∗ p ≤ 0.05), a pairwise comparison was performed and the Bonferroni correction for multiple tests was applied. Data on two-by-two comparison are shown in and .

Article Snippet: 229e full-length spike protein , Sino Biological , Cat#40605-V08B.

Techniques: Infection, Comparison

Journal: Frontiers in Immunology

Article Title: Effect of seasonal coronavirus immune imprinting on the immunogenicity of inactivated COVID-19 vaccination

doi: 10.3389/fimmu.2023.1195533

Figure Lengend Snippet: Seroprevalence of sCoVs and SARS-CoV-2 S IgG antibodies in different cohorts.

Article Snippet: Briefly, to detect corresponding antibodies, 96-well EIA/RIA plates (Corning, #3590) were coated with recombinant SARS-CoV-2 full-length S (Sino Biological, #40591-V08B1-1), SARS-CoV-2 S1(Sino Biological, #40591-V08H), S2 (Sino Biological, #40590-V08B), SARS-CoV-2 full-length N (Sino Biological, #40588-V08B), HCoV-HKU1 full-length S (Sino Biological, #40606-V08B), HCoV-NL63 full-length S (Sino Biological, #40604-V08B), HCoV-OC43 full-length S (Sino Biological, #40607-V08B), and HCoV-229E full-length S proteins (Sino Biological, #40605-V08B) at 100ng/ul in ELISA coating buffer (Solarbio, #C1055) respectively, and incubated at 4°C overnight.

Techniques:

Journal: Cell

Article Title: Seasonal human coronavirus antibodies are boosted upon SARS-CoV-2 infection but not associated with protection

doi: 10.1016/j.cell.2021.02.010

Figure Lengend Snippet: Identification of pre-existing cross-reactive SARS-CoV-2 antibodies in human serum prior to the pandemic (A–C) ELISAs were completed to quantify levels of serum antibodies binding to the SARS-CoV-2 full-length S protein (A), the S-RBD (B), and the N protein (C). Dashed line denotes lower limit of detection (LOD = 50); dotted line represents a threshold set 2-fold above LOD (>100). We tested samples collected from 431 individuals in the summer of 2017, prior to the global pandemic. We also tested samples collected from 15 individuals after confirmed SARS-CoV-2 infections as well as from recovered adults. (D) The relationship between antibody titers in donors with detectable IgG against the S-RBD and/or full-length S is shown. (E) SARS-CoV-2 pseudotype neutralization assays were completed with pre-pandemic serum samples with (n = 14) and without (n = 29) cross-reactive SARS-CoV-2 antibodies, as well as serum samples from individuals after confirmed SARS-CoV-2 infections (n = 15); one-way ANOVA Tukey’s multiple comparisons of log2 transformed antibody titers ∗∗∗∗ p < 0.0001; dotted line denotes lower LOD (=10). (F–H) ELISAs were completed to quantify levels of serum antibodies binding to the full-length S proteins from 229E, NL63, and OC43 with pre-pandemic serum samples with (n = 17) and without (n = 17) cross-reactive SARS-CoV-2 antibodies. Unpaired t tests of log2 transformed antibody titers ∗∗ p < 0.002. Horizontal lines indicate geometric mean and error bars represent standard deviation. See also Figure S1 , Figure S2 , Figure S3 , Figure S4 , and .

Article Snippet: SARS-CoV-2 nucleocapsid (N) protein, and full-length hCoV spike antigens (OC43, 229E, and NL63) were purchased (Sino Biological, Wayne PA; cat. 40588-V08B, 40607-V08B, 40604-V08B, and 40605-V08B, respectively) and reconstituted in Dulbecco’s phosphate buffered saline (DPBS).

Techniques: Binding Assay, Neutralization, Transformation Assay, Standard Deviation

Journal: Cell

Article Title: Seasonal human coronavirus antibodies are boosted upon SARS-CoV-2 infection but not associated with protection

doi: 10.1016/j.cell.2021.02.010

Figure Lengend Snippet: There are no obvious age-related differences in pre-pandemic SARS-CoV-2 and hCoV reactive antibodies, related to Figure 1 ELISAs were completed to measure levels of serum antibodies binding to the SARS-CoV-2 full-length spike (S) protein ( A ), SARS-CoV-2 receptor binding domain (S-RBD) of S ( B ), SARS-CoV-2 nucleocapsid (N) protein ( C ), 229E S protein ( D ), NL63 S protein ( E ), and OC43 S protein ( F ). Serum samples collected from 431 individuals in the summer of 2017 were tested. Reciprocal titer from serially-diluted serum samples ( A-C ) and optical densities at 450nm wavelength (OD 450 ) of 1:500 dilution of serum ( D-F ) are shown. Dashed line denotes lower limit of detection (LOD = 50), dotted line represents a threshold set 2-fold above LOD (> 100).

Article Snippet: SARS-CoV-2 nucleocapsid (N) protein, and full-length hCoV spike antigens (OC43, 229E, and NL63) were purchased (Sino Biological, Wayne PA; cat. 40588-V08B, 40607-V08B, 40604-V08B, and 40605-V08B, respectively) and reconstituted in Dulbecco’s phosphate buffered saline (DPBS).

Techniques: Binding Assay

Journal: Cell

Article Title: Seasonal human coronavirus antibodies are boosted upon SARS-CoV-2 infection but not associated with protection

doi: 10.1016/j.cell.2021.02.010

Figure Lengend Snippet: Pre-pandemic SARS-CoV-2 and OC43-reactive antibodies are not associated with protection from SARS-CoV-2 infection (A and B) We quantified antibody levels in pre-pandemic serum samples collected from individuals who later became SARS-CoV-2 infected (cases; n = 251) and those who did not become SARS-CoV-2 infected (controls; n = 251). ELISAs were completed to quantify levels of antibodies reactive to SARS-CoV-2 proteins (S, S-RBD, and N) and the OC43 S protein. Shown are data using samples collected from the entire cohort between August 2013 and March 2020 (A) and samples from a smaller subset of individuals collected between April 2019 and March 2020 (B). Antibody titers between cases and controls were not significantly different as determined by unpaired t tests of log2 transformed antibody titers. Dashed line denotes lower limit of detection (LOD = 50), dotted line represents a threshold set 2-fold above LOD (>100). See also and .

Article Snippet: SARS-CoV-2 nucleocapsid (N) protein, and full-length hCoV spike antigens (OC43, 229E, and NL63) were purchased (Sino Biological, Wayne PA; cat. 40588-V08B, 40607-V08B, 40604-V08B, and 40605-V08B, respectively) and reconstituted in Dulbecco’s phosphate buffered saline (DPBS).

Techniques: Infection, Transformation Assay

Journal: Cell

Article Title: Seasonal human coronavirus antibodies are boosted upon SARS-CoV-2 infection but not associated with protection

doi: 10.1016/j.cell.2021.02.010

Figure Lengend Snippet: SARS-CoV-2 infections boost antibodies that react to OC43 S protein (A–D) We quantified antibody levels in serum collected from 27 individuals 0 and 7 days after hospitalization for COVID-19. ELISAs were completed to quantify levels of antibodies reactive to the S proteins of 229E, NL63, OC43, and SARS-CoV-2. IgG titers (A) and titer fold change (B) are shown. Levels of OC43 S-reactive antibodies (C) and fold change in OC43 S-reactive antibodies (D) were not associated with disease outcome. Paired t test of log2 transformed antibody titers, ∗∗∗∗ p < 0.0001. One-way ANOVA Tukey’s multiple comparisons fold-change in antibody titers, ∗ p < 0.04. Horizontal lines indicate the median and error bars show interquartile ranges. See also Figure S6 .

Article Snippet: SARS-CoV-2 nucleocapsid (N) protein, and full-length hCoV spike antigens (OC43, 229E, and NL63) were purchased (Sino Biological, Wayne PA; cat. 40588-V08B, 40607-V08B, 40604-V08B, and 40605-V08B, respectively) and reconstituted in Dulbecco’s phosphate buffered saline (DPBS).

Techniques: Transformation Assay

Journal: Cell

Article Title: Seasonal human coronavirus antibodies are boosted upon SARS-CoV-2 infection but not associated with protection

doi: 10.1016/j.cell.2021.02.010

Figure Lengend Snippet: Antibodies directed to the S2 region of OC43 spike are boosted during SARS-CoV-2 infection, related to Figure 3 We quantified antibody levels in serum collected from 27 individuals 0 and 7 days after hospitalization for COVID-19. ELISAs were completed to measure levels of serum antibodies binding to the OC43 full-length spike (FL) protein and the individual S1 and S2 subunits of the OC43 spike. ( A ) IgG titers and ( B ) titer fold change are shown. Paired t test of log2 transformed antibody titers, ∗∗∗∗ p < 0.0001. One-way ANOVA Tukey’s multiple comparisons fold-change in antibody titers, * p < 0.02 ∗∗ p < 0.005. Horizontal lines indicate the median and error bars show interquartile range.

Article Snippet: SARS-CoV-2 nucleocapsid (N) protein, and full-length hCoV spike antigens (OC43, 229E, and NL63) were purchased (Sino Biological, Wayne PA; cat. 40588-V08B, 40607-V08B, 40604-V08B, and 40605-V08B, respectively) and reconstituted in Dulbecco’s phosphate buffered saline (DPBS).

Techniques: Infection, Binding Assay, Transformation Assay

Journal: Cell

Article Title: Seasonal human coronavirus antibodies are boosted upon SARS-CoV-2 infection but not associated with protection

doi: 10.1016/j.cell.2021.02.010

Figure Lengend Snippet:

Article Snippet: SARS-CoV-2 nucleocapsid (N) protein, and full-length hCoV spike antigens (OC43, 229E, and NL63) were purchased (Sino Biological, Wayne PA; cat. 40588-V08B, 40607-V08B, 40604-V08B, and 40605-V08B, respectively) and reconstituted in Dulbecco’s phosphate buffered saline (DPBS).

Techniques: Virus, Generated, Recombinant, Plasmid Preparation, Software